Hypothesis-driven dragging of transcriptomic data to analyze proven targeted pathways in Rhinella arenarum larvae exposed to organophosphorus pesticides

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dc.creator Pires, Natalia S.
dc.creator Lascano, Cecilia
dc.creator Ousset, Julia
dc.creator Ceschin, Danilo G.
dc.creator Venturino, Andrés
dc.date 2022
dc.date.accessioned 2024-04-04T17:44:14Z
dc.date.available 2024-04-04T17:44:14Z
dc.identifier.issn 2045-2322 es_ES
dc.identifier.uri http://rdi.uncoma.edu.ar/handle/uncomaid/17808
dc.description.abstract Transcriptional analysis of the network of transcription regulators and target pathways in exposed organisms may be a hard task when their genome remains unknown. The development of hundreds of qPCR assays, including primer design and normalization of the results with the appropriate housekeeping genes, seems an unreachable task. Alternatively, we took advantage of a whole transcriptome study on Rhinella arenarum larvae exposed to the organophosphorus pesticides azinphos-methyl and chlorpyrifos to evaluate the transcriptional effects on a priori selected groups of genes. This approach allowed us to evaluate the effects on hypothesis-selected pathways such as target esterases, detoxifying enzymes, polyamine metabolism and signaling, and regulatory pathways modulating them. We could then compare the responses at the transcriptional level with previously described effects at the enzymatic or metabolic levels to obtain global insight into toxicity–response mechanisms. The effects of both pesticides on the transcript levels of these pathways could be considered moderate, while chlorpyrifos-induced responses were more potent and earlier than those elicited by azinphos-methyl. Finally, we inferred a prevailing downregulation effect of pesticides on signaling pathways and transcription factor transcripts encoding products that modulate/control the polyamine and antioxidant response pathways. We also tested and selected potential housekeeping genes based on those reported for other species. These results allow us to conduct future confirmatory studies on pesticide modulation of gene expression in toad larvae. es_ES
dc.format application/pdf es_ES
dc.language eng es_ES
dc.publisher Nature Publishing Group es_ES
dc.relation.uri https://doi.org/10.1038/s41598-022-21748-6 es_ES
dc.rights Atribución-NoComercial-CompartirIgual 2.5 Argentina es_ES
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ es_ES
dc.source Scientific Reports 12:17712 es_ES
dc.subject Toxicogenomic es_ES
dc.subject Rhinella arenarum es_ES
dc.subject Biología molecular es_ES
dc.subject.other Ciencias de la Tierra y Medio Ambiente es_ES
dc.subject.other Ciencias Agrarias y Forestales es_ES
dc.title Hypothesis-driven dragging of transcriptomic data to analyze proven targeted pathways in Rhinella arenarum larvae exposed to organophosphorus pesticides es_ES
dc.type Articulo es
dc.type article eu
dc.type acceptedVersion eu
dc.description.fil Fil: Pires, Natalia S. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina. es_ES
dc.description.fil Fil: Pires, Natalia S. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina. es_ES
dc.description.fil Fil: Lascano, Cecilia. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina. es_ES
dc.description.fil Fil: Lascano, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina. es_ES
dc.description.fil Fil: Ousset, Julia. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina. es_ES
dc.description.fil Fil: Ousset, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina. es_ES
dc.description.fil Fil: Ceschin, Danilo G. Centro de Investigación en Medicina Traslacional “Severo R. Amuchástegui; Argentina. es_ES
dc.description.fil Fil: Ceschin, Danilo G. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra ; Argentina. es_ES
dc.description.fil Fil: Venturino, Andrés. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina es_ES
dc.description.fil Fil: Venturino, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina. es_ES
dc.cole Artículos es_ES


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