Biological control of Phytophthora collar rot of pear using regional Trichoderma strains with multiple mechanisms

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dc.creator Sánchez, Aixa Daihana
dc.creator Ousset, María Julia
dc.creator Sosa, María Cristina
dc.date 2019
dc.date.accessioned 2019-12-05T13:02:57Z
dc.date.available 2019-12-05T13:02:57Z
dc.identifier http://rdi.uncoma.edu.ar/handle/uncomaid/15552
dc.identifier.issn 1049-9644 es_ES
dc.description.abstract The Alto Valle of Río Negro is the main exporter and producer region of pear in Argentina, ʻBartlettʼ being the most important cultivar. Phytophthora cactorum and Phytophthora spp. cause significant economic losses in commercial pear production from tree death and weakening and fruit rot. The harmful effect of fungicides and market regulations have created the need to search for promising natural biocontrol agents in integrated crop management programmes. As regional isolates of Trichoderma spp. can be effective biological controllers, Trichoderma was selectively isolated from healthy trees next to trees with collar rot, using Rose Bengal selective medium. All Trichoderma isolates (n = 88) were evaluated against four Phytophthora spp., pathogens of pear by inhibition of mycelia growth (MG) and mycoparasitism. Eighteen isolates reduced the MG of at least two species of Phytophthora by more than 45% and showed mycoparasitism (2 to 4 scale degrees). These isolates were molecularly identified and evaluated in vitro (growth and metabolite production) and in vivo (growth promotion) against P. cactorum. From six isolates selected by PCA, three regional T. harzianum strains with the best antagonistic attributes and PHI K tolerant were evaluated against P. cactorum in a semi commercial bioassay in young pear trees. During the first year of our two-year study, all regional isolates of preventively evaluated Trichoderma spp. decreased the severity of collar rot on pear to a large extent, but without significant differences with the commercial T. atroviride strain and PHI K. Trichoderma harzianum 1330 and 1377 strains preventively reduced pear collar rot by 97% with respect to the diseased control. In the second year, the regional isolates again reached higher biocontrol percentages against P. cactorum. In the curative experiment, regional Trichoderma strains showed no significant differences from PHI K and the commercial isolate. Among all curative and preventive treatments, the regional T. harzianum 1367 strain controlled the rot area caused by P. cactorum by 97%, with the lowest average lesion area (0.11 cm2). es_ES
dc.format application/pdf es_ES
dc.format.extent pp. 124-134 es_ES
dc.language eng en
dc.publisher Editorial Board es_ES
dc.publisher Elsevier es_ES
dc.relation.uri https://www.sciencedirect.com/science/article/pii/S1049964419302993?via%3Dihub es_ES
dc.rights Atribución-NoComercial-CompartirIgual 2.5 Argentina es_ES
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ es_ES
dc.source Biological Control Volume 135, August 2019 es_ES
dc.subject Soil-borne pathogen es_ES
dc.subject Biocontrol es_ES
dc.subject Fungal antagonist es_ES
dc.subject P. cactorum es_ES
dc.subject Pyrus communis es_ES
dc.subject.other Ciencias Agrarias y Forestales es_ES
dc.title Biological control of Phytophthora collar rot of pear using regional Trichoderma strains with multiple mechanisms es_ES
dc.type Articulo es
dc.type article eu
dc.type acceptedVersion eu
dc.description.fil Fil: Sosa, Maria Cristina. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Agentina es_ES
dc.description.fil Fil: Sánchez, Aixa Daihana. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Agentina es_ES
dc.description.fil Fil: Ousset, María Julia. Universidad Nacional del Comahue. CITAAC. CONICET; Agentina es_ES
dc.cole Artículos es_ES


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