https://rdi.uncoma.edu.ar/handle/uncomaid/44 2026-04-17T10:47:08Z 2026-04-17T10:47:08Z https://rdi.uncoma.edu.ar/handle/uncomaid/19298 2026-04-13T14:34:02Z

Susceptibility of placental mitochondria to oxidative stress Background: Two different mitochondrial fractions (MFs) have been character- ized in the human placenta: the “light” and “heavy” fractions (LMF and HMF). Although these organelles are the main source of reactive oxygen species, an imbalance between their production and the rate of detoxification represents a seri- ous threat to mitochondrial homeostasis and, in the case of the placenta, also to the fetus. The aim of this study was to evaluate the antioxidant capacity and suscepti- bility to oxidative stress in both types of MFs. Methods: Human MFs were isolated from healthy donors (n = 11) and either incubated or not with H2O2. Catalase (CAT) activity, and reduced glutathione (GSH), lipid peroxidation (LP), and protein carbonylation (PC) levels were determined. Results: H2O2 treatment increased LP and PC levels and decreased CAT activity. GSH levels were similar in control and treated MFs. Conclusion: H2O2 caused oxidative damage in both LMF and HMF and the antioxidant system measured in these two MFs responded similarly. To the best of our knowledge, this is the first partial description of the antioxidant defense in pla- cental HMF and LMF performed in a cell-free assay. The small number of antioxi- dant system parameters measured did not allow detecting differences between HMF and LMF.

https://rdi.uncoma.edu.ar/handle/uncomaid/19296 2026-04-09T17:37:04Z

Enhancing the efficacy of yeast biocontrol agents against postharvest pathogens through nutrient profiling and the use of other additives Vishniacozyma victoriae and Pichia membranifaciens were selected in a previous work for their biocontrol effec- tiveness against Penicillium expansum and Botrytis cinerea, the causal agents of blue and grey mold of pear fruits. The objective of this study was to determine the effects of different nutrient or additives on the growth of the two antagonist yeasts and of the two fungal pathogens in order to develop a rational selection based on nutritional profiles to be used in biocontrol enhancement of decay in pear fruits. Twenty-six different nutrient sources including 18 amino acids, five sugars, three inorganic nitrogen and one iron source were tested in vitro for their effect on the growth of the two yeasts and the two pathogens. Nutrients that promoted the growth of the yeasts and inhibited the growth of pathogens were applied with the antagonist to wounded fruits to evaluate their effect on enhancing biocontrol in cold storage. In general, the effect of each additive was specific to the yeasts and pathogens used in the in situ assays. The combination of the yeast and some additives resulted in a sig- nificantly higher activity with respect to the single treatments applied separately, producing synergistic effects. The effect of the exogenous application of CaCl2 and chitosan together with the antagonist yeasts was also evaluated against the two pathogens. The most effective mixtures were the CaCl2 with the two antagonist yeasts. Our research demonstrates that manipulating the chemical environment by adding several amino acid and/or Cl2Ca results in improved antagonistic activity of Vishniacozyma victoriae and Pichia membranifaciens against two postharvest diseases of pear fruits.

https://rdi.uncoma.edu.ar/handle/uncomaid/19291 2026-04-06T17:34:42Z

Condiciones laborales y participación sindical de enfermería durante la pandemia de COVID-19 y post pandemia, en el Hospital Bouquet Roldán, Neuquén, periodo 2021-2024 La presente investigación tiene como objetivo analizar las condiciones laborales y la participación sindical del personal de enfermería del Hospital Bouquet Roldán, en Neuquén, durante el período de 2021 a 2024, en el contexto de la pandemia de COVID-19 y su etapa posterior. Se empleará un enfoque descriptivo y de corte transversal, utilizando entrevistas semiestructuradas a una muestra de enfermeros/as y licenciados/as en enfermería que atendieron a pacientes con COVID-19. La intención es recopilar sus experiencias sobre las condiciones de trabajo y su vinculación con la participación sindical. Se concluye que la pandemia provocó una crisis en el Hospital Bouquet Roldán (HBR), evidenciando las precarias condiciones laborales de los trabajadores, caracterizadas por la falta de insumos en general, déficit en la infraestructura, una sobrecarga laboral debido a la escasez de personal y la carencia de capacitaciones adecuadas. El reconocimiento salarial fue insuficiente, lo que impulsó el surgimiento de movimientos autoconvocados que lucharon por reivindicaciones como la mejora salarial y la incorporación de eventuales a planta permanente en el sistema . Sin embargo, estas luchas han perdido vigencia en la actualidad en el HBR.; The present research aims to analyze the working conditions and union participation of nursing staff at the Bouquet Roldán Hospital in Neuquén, from 2021 to 2024, in the context of the Covid- 19 pandemic and its aftermath. A descriptive and cross-sectional approach will be used, employing semi- structured interviews with a sample consisting of Licensed Practical Nurses (LPN) and Registered Nurses (RN) who attended Covid-19 patients. The intention is to gather their experiences regarding working conditions and its relationship with union participation. It is concluded, that the pandemic caused a crisis at the Bouquet Roldán Hospital, evidenced by the employee’s precarious working conditions, characterized by general lack of supplies, infrastructure deficits, work overload due to staff shortages and the absence of adequate training. In addition, the salary recognition was insufficient, which spurred the emersion of self-convened movements that would fight for demands such as salary improvements and the incorporation of temporary staff into permanent positions solidifying the body of the system. However, nowadays, these discussions have lost power and momentum at the hospital.

https://rdi.uncoma.edu.ar/handle/uncomaid/19261 2026-04-01T13:12:12Z

Parasitosis intestinales y su relacion con factores socioeconómicos y condiciones de habitat en niños de Neuquén, Patagonia, Argentina Se investigó la prevalencia y distribución de parásitos intestinales (PI) en niños de dos poblaciones de diferente nivel socioeconómico, ubicadas en la misma zona de la ciudad de Neuquén, con el fin de evaluar su relación con las condiciones del hábitat y factores socioeconómicos. Se analizaron muestras seriadas de heces y raspados anales de 126 niños de entre 2 y 14 años de dos sectores del área suburbana de Neuquén (Sector I y Sector II). Los datos sobre las condiciones del hábitat y los parámetros socioeconómicos se obtuvieron mediante visitas domiciliarias y una encuesta observacional estructurada. Se detectó la presencia de PI en el 50,7% de los niños del Sector I (barrio suburbano con condiciones sanitarias adecuadas y nivel socioeconómico medio o medio bajo) y en el 92,9% de los niños del Sector II (asentamiento marginal con malas condiciones sanitarias y bajo nivel socioeconómico). Se identificaron siete especies de protozoos intestinales y cuatro de helmintos. Blastocystis hominis fue la especie más frecuente en ambas poblaciones. No se encontraron helmintos distintos de Enterobius vermicularis en el Sector I, y la prevalencia de estas especies fue muy baja en el Sector II. Las deficientes condiciones de hábitat y los bajos parámetros socioeconómicos mostraron una relación con una mayor prevalencia de IP de transmisión directa, como protozoos y E. vermicularis, en las poblaciones estudiadas. Sin embargo, incluso en este contexto favorable para la transmisión, las especies parasitarias que requieren etapas intermedias de desarrollo en el suelo no encuentran un hábitat adecuado para su diseminación en esta región.