Characterization of placental cholinesterases and activityinduction associated to environmental organophosphateexposure

Show simple item record

dc.creator Sanchez, Susana es_ES
dc.creator Vera, Berta es_ES
dc.creator Montagna, Cristina es_ES
dc.creator Magnarelli, Gladis es_ES
dc.date 2014-11
dc.date.accessioned 2019-05-30T14:05:43Z
dc.date.available 2019-05-30T14:05:43Z
dc.identifier https://www.journals.elsevier.com/toxicology-reports es_ES
dc.identifier.issn 2214-7500 es_ES
dc.identifier.uri http://rdi.uncoma.edu.ar:8080/handle/123456789/15221
dc.description.abstract Although non-innervated, the placenta contains both cholinesterases (ChEs), butyryl-cholinesterase (BChE) and acetylcholinesterase (AChE). These enzymes are well-known fortheir multiple molecular forms. In a first approach, we used recognized specific inhibitors,substrate preferences and non-denaturating gel electrophoresis in order to characterize theChE profile of term placenta from uncomplicated pregnancy. Results strongly suggest thatthe predominant cholinesterasic form present was tetrameric BChE.It is well established that both ChEs are targets of cholinesterase-inhibiting organophos-phates (OP), one of the most important classes of chemicals actively applied to theenvironment. However, we have previously reported increased ChEs activity in placenta ofrural residents exposed to OP. In the present work, we have studied: 1) whether this find-ing was reproducible and, 2) whether AChE or BChE up regulation is behind the increase ofplacental ChE activity. The population studied included forty healthy women who live inan agricultural area. Samples were collected during both the OP pulverization period (PP)and the recess period (RP). The placental ChEs activity increased in PP, evidencing repro-ducibility of previous results. The analysis of non-denaturating gels revealed that increasedactivity of total ChE activity in placenta from women exposed to OP may be attributable totetrameric BChE up-regulation. es_ES
dc.format application/pdf es_ES
dc.format.extent pp. 437–442 es_ES
dc.language eng en
dc.publisher Elsevier es_ES
dc.publisher Board es_ES
dc.rights Atribución-NoComercial-SinDerivadas 2.5 Argentina *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ *
dc.source Toxicology Reports 2 (2015) es_ES
dc.subject Placenta es_ES
dc.subject Butyrylcholinesterase es_ES
dc.subject Acetylcholinesterase es_ES
dc.subject Organophosphates es_ES
dc.subject.other Ciencias Biomédicas es_ES
dc.title Characterization of placental cholinesterases and activityinduction associated to environmental organophosphateexposure es_ES
dc.type Articulo es
dc.type article eu
dc.type acceptedVersion eu
dc.description.fil Fil: Sanchez, Susana. Universidad Nacional del Comahue. Facultad de Ciencias Médicas; Argentina. es_ES
dc.description.fil Fil: Vera, Berta. Universidad Nacional del Comahue. Facultad de Ciencias Médicas; Argentina. es_ES
dc.description.fil Fil: Montagna, Cristina. Universidad Nacional del Comahue. Facultad de Ingeniería; Argentina. es_ES
dc.description.fil Fil: Magnarelli, Gladis. Universidad Nacional del Comahue. Facultad de Ingeniería. LIBIQUIMA; Argentina. es_ES


Files in this item

This item appears in the following Collection(s)

Show simple item record

Atribución-NoComercial-SinDerivadas 2.5 Argentina Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 2.5 Argentina

Search RDI


Browse

My Account

Statistics